Antagonism of autochthonous lactic acid bacteria from raw milk against Staphylococcus aureus ATCC 12600 and Escherichia coli ATTC 25922
DOI:
https://doi.org/10.19180/1809-2667.v21n32019p443-451Keywords:
Antimicrobial activity, LAB, Inhibitory action, Pathogenic microorganismsAbstract
The objective of this research was to isolate and detect the in vitro antimicrobial effect of autochthonous lactic acid bacteria (LAB) isolated from raw milk from Bom Jesus do Itabapoana (Brazil) against Staphylococcus aureus ATCC 12600 and Escherichia coli ATTC 25922. The raw milk samples were counted and isolated by pour plate technique on MRS and M17 agar. LAB isolates were confirmed after catalase test and Gram staining. The antimicrobial activity step consisted of the overlayed test by spread plate on MRS and M17 agar and, above, the TSA medium with the inoculated pathogen. Ten samples were analyzed, in which 55% of the counting results prevailed at 4 log CFU/mL of the 95 isolated strains, 74 presented LAB characteristics, and of these, 37 were evaluated for their antagonistic activities on S. aureus and E. coli. Approximately 76% of the isolates used for antimicrobial activity against S. aureus and E. coli showed inhibitory action against at least one of the pathogens tested. As far as it is known, this is the first study involving autochthonous LAB strains from milk in northwestern Rio de Janeiro.Downloads
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